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, 16 (4), 370-5

Genetic Engineering of Proteins With Cell Membrane Permeability

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Genetic Engineering of Proteins With Cell Membrane Permeability

M Rojas et al. Nat Biotechnol.

Abstract

The discovery of methods for generating proteins with inherent cell membrane-translocating activity will expand our ability to study and manipulate various intracellular processes in living systems. We report a method to engineer proteins with cell-membrane permeability. After a 12-amino acid residue membrane-translocating sequence (MTS) was fused to the C-terminus of glutathione S-transferase (GST), the resultant GST-MTS fusion proteins were efficiently imported into NIH 3T3 fibroblasts and other cells. To explore the applicability of this nondestructive import method to the study of intracellular processes, a 41-kDa GST-Grb2SH2-MTS fusion protein containing the Grb2 SH2 domain was tested for its effect on the epidermal growth factor (EGF)-stimulated signaling pathway. This fusion protein entered cells, formed a complex with phosphorylated EGF receptor (EGFR), and inhibited EGF-induced EGFR-Grb2 association and mitogen-activated protein kinase activation.

Comment in

  • Ferrying proteins to the other side.
    Fernandez T, Bayley H. Fernandez T, et al. Nat Biotechnol. 1998 May;16(5):418-20. doi: 10.1038/nbt0598-418. Nat Biotechnol. 1998. PMID: 9592386 No abstract available.

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