Anandamide, an endogenous cannabinoid receptor ligand, also interacts with 5-hydroxytryptamine (5-HT) receptor

Biol Pharm Bull. 1998 Mar;21(3):224-6. doi: 10.1248/bpb.21.224.


Interactions of anandamide (N-arachidonylethanolamide), an endogenous compound for cannabinoid receptors, with the receptors for 5-hydroxytryptamine (5-HT), benzodiazepine, and gamma-aminobutyric acid(A) (GABA[A]) receptors in bovine synaptic membrane were examined. Anandamide decreased the 5-HT receptor bindings at concentrations of 1-100 microM, although it did not cause any change in benzodiazepine or GABA(A) receptor bindings. A high concentration of anandamide, 100 microM, significantly decrease both [3H]5-HT and [3H]ketanserin bindings. The present study revealed that the pharmacological activity of anandamide might be partially mediated through the 5-HT receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acids / metabolism*
  • Brain / metabolism
  • Cattle
  • Endocannabinoids
  • Ligands
  • Polyunsaturated Alkamides
  • Protein Binding
  • Receptors, Cannabinoid
  • Receptors, Drug / metabolism*
  • Receptors, GABA / metabolism
  • Receptors, Serotonin / metabolism*


  • Arachidonic Acids
  • Endocannabinoids
  • Ligands
  • Polyunsaturated Alkamides
  • Receptors, Cannabinoid
  • Receptors, Drug
  • Receptors, GABA
  • Receptors, Serotonin
  • anandamide