Abstract
At least eight conserved motifs are visible in the totivirus RNA-dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiae double-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Capsid / genetics
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Capsid / metabolism
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Conserved Sequence*
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Molecular Sequence Data
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Mutagenesis
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RNA-Dependent RNA Polymerase / genetics
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RNA-Dependent RNA Polymerase / metabolism*
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Saccharomyces cerevisiae / virology*
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Satellite Viruses / physiology
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Totiviridae / enzymology*
Substances
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RNA-Dependent RNA Polymerase