Identification of a full-length cDNA for an endogenous retrovirus of miniature swine

J Virol. 1998 May;72(5):4503-7. doi: 10.1128/JVI.72.5.4503-4507.1998.


Endogenous retroviruses of swine are a concern in the use of pig-derived tissues for xenotransplantation into humans. The nucleotide sequence of porcine endogenous retrovirus taken from lymphocytes of miniature swine (PERV-MSL) has been characterized. PERV-MSL is a type C retrovirus of 8,132 bp with the greatest nucleic acid sequence identity to gibbon ape leukemia virus and murine leukemia virus. Constitutive production of PERV-MSL RNA has been detected in normal leukocytes and in multiple organs of swine. The copy numbers of full-length PERV sequences per genome (approximately 8 to 15) vary among swine strains. The open reading frames for gag, pol, and env in PERV-MSL have over 99% amino acid sequence identity to those of Tsukuba-1 retrovirus and are highly homologous to those of endogenous retrovirus of cell line PK15 (PK15-ERV). Most of the differences in the predicted amino acid sequences of PK15-ERV and PERV-MSL are in the SU (cell attachment) region of env. The existence of these PERV clones will enable studies of infection by endogenous retroviruses in xenotransplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • DNA, Viral / analysis*
  • Gene Dosage
  • Gene Expression
  • Gene Library
  • Genes, gag
  • Genes, pol
  • Molecular Sequence Data
  • Proviruses / genetics
  • Retroviridae / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Swine
  • Swine, Miniature / virology*


  • DNA, Complementary
  • DNA, Viral

Associated data

  • GENBANK/AF038599
  • GENBANK/AF038600
  • GENBANK/AF038601