Characterization of CKbeta8 and CKbeta8-1: Two Alternatively Spliced Forms of Human Beta-Chemokine, Chemoattractants for Neutrophils, Monocytes, and Lymphocytes, and Potent Agonists at CC Chemokine Receptor 1

Blood. 1998 May 1;91(9):3118-26.

Abstract

Two new members of human beta-chemokine cDNA were isolated based on structural and functional similarities to human leukotactin-1. One of these clones was identical to the previously isolated human beta-chemokine, CKbeta8, whereas the other is a splicing variant of CKbeta8, therefore named CKbeta8-1. CKbeta8 was short in 51 nucleotides (17 amino acids) compared with CKbeta8-1. The mature proteins of CKbeta8-1 and CKbeta8 consisted of 116 and 99 amino acids with calculated molecular weights of 12,500 and 10,950, respectively. Both CKbeta8-1 and CKbeta8 were potent agonists at CCR1. These chemokines chemoattracted neutrophils, monocytes, and lymphocytes. They also significantly suppressed colony formation by human bone marrow, granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. To our knowledge, this is the first example that an alternative splicing produces two active beta-chemokines from a single gene.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Base Sequence
  • Calcium / physiology
  • Cells, Cultured
  • Chemokines, CC / genetics*
  • Chemotaxis, Leukocyte
  • Chromosomes, Human, Pair 17
  • DNA, Complementary / genetics
  • Exons
  • Genes
  • Humans
  • Introns
  • Lymphocytes / physiology
  • Molecular Sequence Data
  • Monocytes / physiology
  • Neutrophils / physiology
  • Receptors, Chemokine / physiology
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Chemokines, CC
  • DNA, Complementary
  • Receptors, Chemokine
  • Calcium

Associated data

  • GENBANK/U58913
  • GENBANK/U67128