Glucagon-like peptide-1 receptor expression in Xenopus oocytes stimulates inositol trisphosphate-dependent intracellular Ca2+ mobilization

FEBS Lett. 1998 Mar 27;425(2):277-80. doi: 10.1016/s0014-5793(98)00254-3.


The signal transduction pathway of the cloned human glucagon-like peptide-1 (GLP-1) receptor was studied in voltage-clamped Xenopus oocytes. Binding of GLP-1(7-36)amide was associated with cAMP production, increased [Ca2+]i and activation of Ca2+-dependent Cl- current. The effect of GLP-1(7-36)amide reflects intracellular Ca2+ mobilization and was suppressed by injection of the Ca2+ chelator BAPTA and the inositol trisphosphate receptor antagonist heparin. The responses were not mimicked by the adenylate cyclase activator forskolin and unaffected by the protein kinase A (PKA) inhibitor Rp-cAMPS. We conclude that GLP-1 receptor expression in Xenopus oocytes evokes inositol trisphosphate-dependent intracellular Ca2+ mobilization independent of the cAMP/PKA signaling pathway.

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Animals
  • Calcium / metabolism*
  • Chloride Channels / physiology
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Gene Expression
  • Glucagon-Like Peptide-1 Receptor
  • Humans
  • Inositol 1,4,5-Trisphosphate / metabolism*
  • Intracellular Fluid
  • Oocytes / metabolism
  • Receptors, Glucagon / genetics
  • Receptors, Glucagon / metabolism*
  • Xenopus laevis / metabolism


  • Chloride Channels
  • GLP1R protein, human
  • Glucagon-Like Peptide-1 Receptor
  • Receptors, Glucagon
  • Inositol 1,4,5-Trisphosphate
  • Cyclic AMP-Dependent Protein Kinases
  • Adenylyl Cyclases
  • Calcium