Comparison between enzyme-linked immunosorbent assay, indirect fluorescent antibody and rapid conglutination tests in detecting antibodies against Babesia bovis

Vet Parasitol. 1998 Jan 31;74(2-4):101-8. doi: 10.1016/s0304-4017(97)00171-4.


The performance of an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFAT) and a rapid conglutination test (RCT) for the detection of antibodies against Babesia bovis, was evaluated with 462 cattle sera from Bahia State; Brazil. The results showed a concordance of 96.6% between the ELISA and IFAT, 90.5% between the ELISA and RCT, and 91.8% between the IFAT and RCT. Although the prevalence rates determined by ELISA (97.2%) and IFAT (96.8%) were higher than that indicated by the RCT (92.9%), performances of the three serological tests were very similar and characterized the region studied as enzootically stable to B. bovis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Protozoan / blood*
  • Babesia bovis / immunology*
  • Babesiosis / diagnosis*
  • Babesiosis / epidemiology
  • Babesiosis / prevention & control
  • Brazil / epidemiology
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / epidemiology
  • Cattle Diseases / prevention & control
  • Complement Fixation Tests / veterinary*
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Female
  • Fluorescent Antibody Technique, Indirect / veterinary*
  • Prevalence
  • Sensitivity and Specificity


  • Antibodies, Protozoan