Solid-phase microextraction of polychlorinated biphenyls

J Chromatogr A. 1998 Mar 27;800(2):257-66. doi: 10.1016/s0021-9673(97)01115-1.


The extraction and analysis of 21 polychlorinated biphenyls (PCBs) ranging from di- to decachlorobiphenyls in ocean, wetland and leachate water samples were achieved using solid-phase microextraction (SPME) with a 100-micron poly(di-methylsiloxane) (PDMS) fiber and gas chromatography-electron-capture detection (GC-ECD). Severe carryover between samples (e.g., 20%) occurs on both stir bars and the SPME fibers demonstrating that it is important to use a new stir bar for each sample, as well as to perform SPME-GC blanks between samples to avoid quantitative errors. The equilibrium partitioning coefficients of individual PCB congeners between PDMS and water were found to be surprisingly different compared to their octanol-water partitioning coefficient (Kow), demonstrating that Kow cannot be used to estimate the partitioning behavior of PCBs in the SPME process. Using a 15-min SPME extraction, SPME analysis with GC-ECD was linear (r2 > or = 0.97) from approximately 5 pg/ml to the solubility limit of each congener. Concentrations in water samples obtained by 15-min SPME extractions compared favorably with those obtained by toluene extractions, demonstrating that SPME combined with GC is a useful technique for the rapid determination of PCBs in water samples.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Absorption
  • Chromatography, Gas / methods*
  • Dimethylpolysiloxanes / chemistry*
  • Environmental Pollutants / analysis*
  • Linear Models
  • Osmolar Concentration
  • Polychlorinated Biphenyls / chemistry*
  • Sensitivity and Specificity
  • Silicones / chemistry*
  • Wetting Agents / chemistry*


  • Dimethylpolysiloxanes
  • Environmental Pollutants
  • Silicones
  • Wetting Agents
  • baysilon
  • Polychlorinated Biphenyls