Cellular and transcriptional changes during excystation of Giardia lamblia in vitro

Exp Parasitol. 1998 Mar;88(3):172-83. doi: 10.1006/expr.1998.4246.


Excystation of Giardia lamblia entails differentiation of dormant cysts into parasitic trophozoites. Despite its importance for infection, this transformation is not understood at the cellular or molecular levels. In these studies, we report that excystation entails detection of environmental stimuli across the tough extracellular cyst wall leading to highly coordinated physiological, structural, and molecular responses. We found that novel cytoplasmic rearrangements and changes in individual species of mRNA and in cytoplasmic pH occur within the cyst wall in the earliest stage of excystation, in response to conditions modeling cyst ingestion and passage into the human stomach. This suggests that cysts do not contain all the mRNA needed for excystation and emergence and supports our hypothesis that external stimuli, including hydrogen ions, may penetrate or be perceived across the cyst wall. In contrast, changes in cyst wall structure or proteins were detected only later in excystation, in the stage that models passage into the human small intestine, where trophozoites can emerge and survive. These findings show that excystation of G. lamblia is a highly complex and active process and provide important insights into its cellular and molecular components.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Giardia lamblia / genetics
  • Giardia lamblia / physiology*
  • Giardia lamblia / ultrastructure
  • Glycoproteins / chemistry
  • Glycoproteins / metabolism
  • Humans
  • Hydrogen-Ion Concentration
  • Intestine, Small / chemistry
  • Microscopy, Electron
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / metabolism
  • RNA, Messenger / metabolism
  • Stomach / chemistry
  • Transcription, Genetic*
  • Up-Regulation


  • Glycoproteins
  • Protozoan Proteins
  • RNA, Messenger