Whole-killed gp120-depleted HIV-1 Antigen in a Murine Model for Prophylactic Vaccination

Vaccine. 1998 Apr;16(7):727-31. doi: 10.1016/s0264-410x(97)00256-9.

Abstract

In this study, the effects were examined of dose and adjuvant of whole-killed gp120-depleted HIV-1 antigen on antibody and cytokine responses in a murine model. Immunization with increasing doses of inactivated HIV-1 antigen in Incomplete Freund's Adjuvant (IFA) resulted in increased production of IL-4 and IgG1 antibody with decreased production of interferon gamma. Immunization with inactivated HIV-1 antigen in Detox PC adjuvant produced TH1 type predominant cytokine patterns along with IgG2a subclass antibody. Higher levels of interferon gamma were associated with immunization with inactivated HIV-1 antigen in Detox PC compared with inactivated HIV-1 in IFA or inactivated HIV-1 in saline. Inactivated HIV-1 antigen in Detox PC adjuvant produced a trend of lower levels of the beta-chemokine MIP-1 alpha compared with inactivated HIV-1 in IFA or saline. Dose and adjuvant play an important role in the type of immune response elicited to a whole-killed HIV vaccine. Low doses of inactivated HIV-1 antigen in Detox PC adjuvant are currently being studied in animal models in order to optimize cell-mediated immunity against HIV infection.

MeSH terms

  • AIDS Vaccines / immunology*
  • AIDS Vaccines / pharmacology*
  • Animals
  • Chemokine CCL4
  • Dose-Response Relationship, Drug
  • Freund's Adjuvant / immunology
  • Freund's Adjuvant / pharmacology*
  • HIV Antibodies / biosynthesis
  • HIV Antigens / immunology*
  • HIV Antigens / pharmacology*
  • HIV Envelope Protein gp120 / immunology*
  • HIV Infections / prevention & control*
  • Immunoglobulin G / biosynthesis
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / immunology
  • Interleukin-4 / biosynthesis
  • Interleukin-4 / immunology
  • Macrophage Inflammatory Proteins / biosynthesis
  • Macrophage Inflammatory Proteins / immunology
  • Mice
  • Mice, Inbred C57BL
  • Vaccines, Inactivated / immunology
  • Vaccines, Inactivated / pharmacology

Substances

  • AIDS Vaccines
  • Chemokine CCL4
  • HIV Antibodies
  • HIV Antigens
  • HIV Envelope Protein gp120
  • Immunoglobulin G
  • Macrophage Inflammatory Proteins
  • Vaccines, Inactivated
  • Interleukin-4
  • Interferon-gamma
  • Freund's Adjuvant