Measurement of 25-hydroxycholecalciferol in the bovine

J Dairy Sci. 1976 Aug;59(8):152204. doi: 10.3168/jds.s0022-0302(76)84396-2.


Bovine plasma 25-hydroxycholecalciferol was estimated by rapid protein binding assay. Serum-binding proteins in the assay were from rats deficient in vitamin D and from normal rats; no differences existed in standard curves. Nanograms 25-hydroxycholecalciferol in eight cows averaged 45.5 +/- 12.2 ml of plasma. Results from the rapid protein binding assay compared favorably (greater than 10% deviation) with values from a 25-hydroxycholecalciferol assay involving high pressure liquid chromatography.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cattle
  • Female
  • Hydroxycholecalciferols / blood*
  • Male
  • Protein Binding
  • Rats
  • Serum Globulins / metabolism
  • Vitamin D Deficiency / blood


  • Hydroxycholecalciferols
  • Serum Globulins