Studies on the antinociceptive effects of sodium nitroprusside and molsidomine in mice

Pol J Pharmacol. Nov-Dec 1997;49(6):395-400.

Abstract

The participation of nitric oxide (NO) in antinociceptive activity of molsidomine and sodium nitroprusside (SNP) was studied in mice using the writhing test. Molsidomine (300 and 150 mg/kg) and SNP (1.52-0.38 mg/kg) induced antinociception that was antagonized by naloxone. L-arginine (500-62.5 mg/kg) did not produce antinociceptive effects, whereas N omega-nitro-L-arginine methyl ester (L-NAME) (37.5-150 mg/kg) induced antinociception which was suppressed by naloxone. Methylene blue did not change the molsidomine- and SNP-induced antinociception, but significantly intensified that produced by L-NAME. L-arginine increased antinociceptive effect of molsidomine but not that of SNP. Antinociceptive activity of L-NAME was partially reversed by L-arginine. D-arginine failed to influence these effects. The present findings suggest that the NO-cGMP pathway is not involved in the mechanism of molsidomine- and SNP-induced antinociception in the writhing test in mice.

MeSH terms

  • Analgesics, Non-Narcotic / pharmacology*
  • Animals
  • Arginine / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Male
  • Methylene Blue / pharmacology
  • Mice
  • Molsidomine / pharmacology*
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Nitric Oxide / biosynthesis
  • Nitric Oxide / physiology*
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitroprusside / pharmacology*
  • Pain Measurement

Substances

  • Analgesics, Non-Narcotic
  • Enzyme Inhibitors
  • Nitroprusside
  • Nitric Oxide
  • Arginine
  • Molsidomine
  • Nitric Oxide Synthase
  • Methylene Blue
  • NG-Nitroarginine Methyl Ester