Epithelial cell proliferation in the digestive tract induced by space restriction and water-immersion stress

Cancer Lett. 1998 Mar 13;125(1-2):141-8. doi: 10.1016/s0304-3835(97)00504-1.


The effects of space restriction and water-immersion stress on epithelial cell proliferation in the digestive tract, with special attention to the esophagus, stomach and duodenum, in 8-week-old SD male rats were examined. Histological assessment revealed spotted hemorrhagic lesions in the fundus of the glandular stomach, accompanied by statistically increased 5-bromo-2'-deoxyuridine (BrdU) labeling index in the fundic and pyloric regions. Furthermore, biochemical analysis demonstrated an increased activity of ornithine decarboxylase (ODC) and spermidine/spermine N1-acetyltransferase (SAT), known as key late-limiting enzymes of the polyamine pathway, in the gastric fundus. The stress may induce a remarkable increase in expression of c-fos, c-jun and c-myc mRNAs in both fundic and pyloric regions of the glandular stomach. There were no remarkable changes in the esophagus. These results indicate that space restriction and water-immersion stress induced cell proliferation in the glandular stomach through overexpression of proto-oncogenes and increased ODC and SAT activities that might be related to the promotion of gastric carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / metabolism
  • Adrenocorticotropic Hormone / blood
  • Animals
  • Apoptosis
  • Bromodeoxyuridine / metabolism
  • Cell Division
  • Duodenum / pathology*
  • Epithelial Cells
  • Esophagus / pathology*
  • Male
  • Ornithine Decarboxylase / metabolism
  • Proto-Oncogenes
  • Rats
  • Rats, Sprague-Dawley
  • Stomach / pathology*
  • Stress, Physiological / pathology*


  • Adrenocorticotropic Hormone
  • Acetyltransferases
  • diamine N-acetyltransferase
  • Ornithine Decarboxylase
  • Bromodeoxyuridine