Embryonic Implantation in Galectin 1/galectin 3 Double Mutant Mice

Dev Dyn. 1998 Apr;211(4):306-13. doi: 10.1002/(SICI)1097-0177(199804)211:4<306::AID-AJA2>3.0.CO;2-L.

Abstract

Galectin 1 and galectin 3 are first expressed in the trophectoderm cells of the implanting embryo and have been implicated in the process of implantation. However, we had previously shown that the lack of galectin 1 in galectin 1 null mutant mice is compatible with implantation. In this study, we describe the generation of galectin 3 null mutant mice and show that they are viable and have no overt abnormalities. The importance of galectin 1 and galectin 3 in implantation was assessed by obtaining double mutant mice [gal1 -/-; gal3 -/-]. We find that implantation can still occur in the absence of both galectin 1 and galectin 3. However, we show that galectin 5, a third member of this gene family, is also present in the blastocyst at the time of implantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation / genetics
  • Antigens, Differentiation / metabolism
  • Antigens, Differentiation / physiology*
  • Blastocyst / metabolism
  • Cells, Cultured
  • Cloning, Molecular
  • Embryo Implantation / physiology*
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Galectin 1
  • Galectin 3
  • Galectins*
  • Hemagglutinins / metabolism
  • Hemagglutinins / physiology*
  • Lectins / metabolism
  • Lectins / physiology
  • Mice
  • Mice, Knockout
  • Microscopy, Confocal

Substances

  • Antigens, Differentiation
  • Galectin 1
  • Galectin 3
  • Galectins
  • Hemagglutinins
  • Lectins
  • galectin 5