Alimentary lipemia enhances the membrane expression of platelet P-selectin without affecting other markers of platelet activation

Atherosclerosis. 1998 Mar;137(1):107-13. doi: 10.1016/s0021-9150(97)00260-8.

Abstract

The present study was conducted to determine whether alimentary lipemia alters platelet activity in vivo. Normolipidemic volunteers were given a fatty meal and platelet function was assessed before, and 3 and 6 h after the meal. Platelet aggregability and secretion was determined using whole blood flow cytometry (expression of platelet P-selectin and fibrinogen binding), filtragometry ex vivo (reflecting platelet aggregability in vivo) and by measurements of platelet specific products in plasma (beta-thromboglobulin and platelet factor 4). Plasma triglycerides increased from 0.8 (0.6:1.1; median, 25th and 75th percentiles) to 1.7 (1.0:2.3) mmol/l at 3 h and returned to baseline after 6 h (P < 0.001, one-way ANOVA). Apo B-100 and apo B-48 were both markedly increased 3 h postprandially in the Sf 60-400 fraction (large VLDLs, P < 0.001 for both), whereas the Sf 20-60 (small VLDLs) and Sf 12-20 fractions (IDL) did not change. The platelet function assessments revealed that the percentage of platelets expressing P-selectin increased by 40% (5%; 64%) after 3 h and by 51% (- 7%; 85%) 6 h postprandially in unstimulated samples (P < 0.05 for both). In samples stimulated by ADP in vitro P-selectin expression increased by 45% (6%; 58%) after 3 h and by 30% (12%; 58%) (P<0.01 for both) after 6 h at 0.1 microM. Platelet P-selectin expression was less influenced at higher ADP concentrations. The plasma levels of beta-thromboglobulin (approximately 20 ng/ml) and platelet factor 4 (approximately 0.3 ng/ml) were not affected by the fat load. Flow cytometric analyses of fibrinogen binding and filtragometry measurements also failed to reveal any postprandial alterations. The present finding of enhanced platelet P-selectin expression suggests that platelets are mildly sensitized postprandially. Whether this is of importance for thrombus formation and atherosclerosis needs to be studied further.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoproteins B / blood
  • Biomarkers / blood
  • Blood Platelets / chemistry
  • Blood Platelets / physiology
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Data Interpretation, Statistical
  • Diet, Atherogenic
  • Dietary Fats / metabolism
  • Humans
  • Hyperlipidemias / physiopathology
  • Hypertriglyceridemia / physiopathology*
  • Lipids / blood
  • Lipoproteins / administration & dosage
  • Lipoproteins / blood
  • Lipoproteins / pharmacology
  • Male
  • P-Selectin / biosynthesis*
  • Platelet Activation / physiology*
  • Platelet Factor 4 / analysis
  • Postprandial Period / physiology
  • Time Factors
  • Triglycerides / blood
  • Triglycerides / physiology*
  • beta-Thromboglobulin / analysis

Substances

  • Apolipoproteins B
  • Biomarkers
  • Dietary Fats
  • Lipids
  • Lipoproteins
  • P-Selectin
  • Triglycerides
  • beta-Thromboglobulin
  • Platelet Factor 4