Rapid method for isolation of total RNA from eukaryotic cell lines and leukocytes

DNA Cell Biol. 1998 Apr;17(4):321-3. doi: 10.1089/dna.1998.17.321.


Total RNA was isolated from human leukocytes (monocytes, granulocytes), various cell lines (COS-7, Mono-Mac-6, L-132, HaCaT, EA.hy926, HL-60), and fungal mycelium by a rapid two-step method. Cells were lysed with NaDodSO4 in a citric acid-containing buffer. This procedure was succeeded by salt precipitation to remove contaminating DNA and protein and a final alcohol precipitation of RNA. Isolated RNA was of high quality, with a reasonable yield and little or no protein or DNA contamination. We present here a fast method for preparing RNA particularly from cell lines, which limits the use of toxic compounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Cell Line
  • Eukaryotic Cells / chemistry*
  • Fungi / chemistry
  • Humans
  • Leukocytes / chemistry*
  • RNA / isolation & purification*


  • RNA