Nasal immunization of humans with dehydrated liposomes containing Streptococcus mutans antigen

Oral Microbiol Immunol. 1997 Dec;12(6):329-35. doi: 10.1111/j.1399-302x.1997.tb00734.x.


Five healthy female adult volunteers were intranasally immunized twice (7-day interval) with 250 micrograms of a crude glucosyltransferase (GTF) preparation from Streptococcus mutans in liposomes. Parotid saliva, nasal wash, and serum were collected prior to and at weekly intervals for 6 weeks following the first immunization for analysis of anti-GTF activity by enzyme-linked immunosorbent assay. The levels of IgA1 anti-GTF activity increased in the nasal wash from all five individuals after immunization. Increases in salivary IgA1 and IgA2 anti-GTF activities were observed to a lesser extent. Increased serum IgM and IgA (but not IgG) anti-GTF activities were seen in immunized subjects. Nasal immunization with a dehydrated liposome-protein vaccine was effective in inducing an apparent secretory IgA antibody response, which was primarily of the IgA1 subclass. These results provide the first evidence of the effective use of a nasal liposome-protein vaccine in humans.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Intranasal
  • Adult
  • Antibodies, Bacterial / analysis
  • Antigens, Bacterial / administration & dosage*
  • Antigens, Bacterial / immunology
  • Antigens, Bacterial / isolation & purification
  • Bacterial Vaccines / administration & dosage*
  • Bacterial Vaccines / immunology
  • Bacterial Vaccines / isolation & purification
  • Desiccation
  • Drug Carriers
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunization / methods*
  • Liposomes
  • Saliva / immunology
  • Streptococcus mutans / immunology*
  • Time Factors


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Vaccines
  • Drug Carriers
  • Liposomes