Free methionine supplementation limits alcohol-induced liver damage in rats

Alcohol Clin Exp Res. 1998 Apr;22(2):352-8. doi: 10.1111/j.1530-0277.1998.tb03660.x.


Alcohol feeding to rats that were submitted to a jejunoileal bypass operation has been shown to result in liver damage being comparable with alcohol-induced liver disease in man. In the present study, a striking effect of free methionine consumption on histological liver injury, triglyceride accumulation, and energy-rich nucleoside content in the liver of rats with a jejunoileal bypass is demonstrated. The animals obtained 0, 30, and 120 mg of methionine in the control group and 0, 30, 120, and 240 mg in the alcohol-fed group per day and per kilogram of body weight for 12 weeks. Methionine was found to strongly improve the alcohol-induced histological changes in the liver. Triglyceride content of the liver was found to decrease in a dose-dependent manner with increasing methionine ingestion (from 255 +/- 20.7 to 49.7 +/- 6.1 micromol/g of protein in the control group and from 233 +/- 17.3 to 42.1 +/- 7.2 micromol/g of protein in the alcohol group). Hepatic adenosine triphosphate content increased significantly with higher methionine consumption (13.5 +/- 0.8 vs. 26.9 +/- 2.8 micromol/g of protein in the control group and 11.9 +/- 1.4 vs. 20.5 +/- 2.5 micromol/g of protein in the alcohol group), whereas no differences were found in the protein and DNA content of the liver. These results underscore the impairment of the transmethylation/transsulfuration pathway in the development of alcohol-induced liver diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine Nucleotides / metabolism
  • Animals
  • Dose-Response Relationship, Drug
  • Energy Metabolism / drug effects
  • Fatty Liver, Alcoholic / pathology
  • Liver / drug effects
  • Liver / pathology
  • Liver Diseases, Alcoholic / pathology*
  • Male
  • Methionine / pharmacology*
  • Rats
  • Rats, Wistar
  • Triglycerides / metabolism


  • Adenine Nucleotides
  • Triglycerides
  • Methionine