Expression of macrophage-derived chemokine (MDC) mRNA in macrophages is enhanced by interleukin-1beta, tumor necrosis factor alpha, and lipopolysaccharide

J Leukoc Biol. 1998 May;63(5):606-11. doi: 10.1002/jlb.63.5.606.

Abstract

A cDNA encoding the C-C chemokine MDC was isolated from a human macrophage cDNA library by differential hybridization using monocyte- and macrophage-specific cDNA probes. During monocyte to macrophage differentiation in vitro, MDC expression is first detected after 1 day of culturing and reaches maximum levels after 6 days when macrophages have fully matured, as judged from the expression of known macrophage marker genes. Exposure of macrophages to lipopolysaccharide (LPS) results in a dose-dependent increase in MDC mRNA levels, with maximum induction occurring after 6-8 h, whereas expression levels of macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-2, interleukin-1beta (IL-1beta), and tumor necrosis factor alpha (TNF-alpha) respond much faster to LPS. Furthermore, MDC expression in macrophages is enhanced by the inflammatory mediators TNF-alpha and IL-1beta. Similar to other TNF-alpha/IL-1beta-inducible genes, costimulation of macrophages with both cytokines leads to higher MDC expression levels than stimulation with a single cytokine. By contrast, both resting and activated monocytes do not express MDC mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemokine CCL22
  • Chemokines, CC / genetics*
  • DNA, Complementary / genetics
  • HL-60 Cells
  • Humans
  • Interleukin-1 / pharmacology*
  • Lipopolysaccharides / pharmacology*
  • Macrophage Activation
  • Macrophages / physiology*
  • Monocytes / physiology
  • RNA, Messenger / genetics
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • CCL22 protein, human
  • Chemokine CCL22
  • Chemokines, CC
  • DNA, Complementary
  • Interleukin-1
  • Lipopolysaccharides
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha