Differential roles of GATA-1 and GATA-2 in growth and differentiation of mast cells

Genes Cells. 1998 Jan;3(1):39-50. doi: 10.1046/j.1365-2443.1998.00166.x.


Background: While mast cells have been previously shown to express both GATA-1 and GATA-2 mRNAs, individual functions for these related factors during their course of differentiation within the mast cell lineage have not yet been defined. To address this question, the expression of GATA-1 and GATA-2 mRNAs and proteins were examined in three mouse mast cell progenitor lines as well as in mast cells isolated from both wild-type and GATA-1-deficient mice.

Results: Both mast cell progenitor lines, as well as primary mouse bone marrow-derived mast cells (BMMCs) and peritoneal mast cells (PMCs) were examined by RNA blotting and immunological analyses. GATA-2 protein was abundantly expressed in all three mast cell lines and in BMMCs, but only weakly in some of PMCs. In contrast, GATA-1 protein was expressed in PMCs and BMMCs after culture in the presence of IL3 and SCF. We also found the presence of Alcian blue staining-positive but berberine staining-negative mast cells in the skin of mice heterozygous to GATA-1 knock-down allele.

Conclusion: These results suggest that the expression of GATA factor-dependent genes is regulated by GATA-2 during mast cell development and that GATA-1 is required for the specification of differentiated mast cell phenotypes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation / physiology*
  • Cell Division / physiology*
  • DNA-Binding Proteins / metabolism*
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA2 Transcription Factor
  • Gene Expression Regulation, Developmental / genetics*
  • Heterozygote
  • Histamine / analysis
  • Histocytochemistry
  • Interleukin-3 / pharmacology
  • Mast Cells / physiology*
  • Mice
  • Mice, Knockout
  • RNA, Messenger / metabolism
  • Stem Cell Factor / pharmacology
  • Stem Cells / physiology
  • Transcription Factors / metabolism*


  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA2 Transcription Factor
  • Gata1 protein, mouse
  • Gata2 protein, mouse
  • Interleukin-3
  • RNA, Messenger
  • Stem Cell Factor
  • Transcription Factors
  • Histamine