Specificity in the interaction of HVA Ca2+ channel types with Ca2+-dependent AHPs and firing behavior in neocortical pyramidal neurons

J Neurophysiol. 1998 May;79(5):2522-34. doi: 10.1152/jn.1998.79.5.2522.


Intracellular recordings and organic and inorganic Ca2+ channel blockers were used in a neocortical brain slice preparation to test whether high-voltage-activated (HVA) Ca2+ channels are differentially coupled to Ca2+-dependent afterhyperpolarizations (AHPs) in sensorimotor neocortical pyramidal neurons. For the most part, spike repolarization was not Ca2+ dependent in these cells, although the final phase of repolarization (after the fast AHP) was sensitive to block of N-type current. Between 30 and 60% of the medium afterhyperpolarization (mAHP) and between approximately 80 and 90% of the slow AHP (sAHP) were Ca2+ dependent. Based on the effects of specific organic Ca2+ channel blockers (dihydropyridines, omega-conotoxin GVIA, omega-agatoxin IVA, and omega-conotoxin MVIIC), the sAHP is coupled to N-, P-, and Q-type currents. P-type currents were coupled to the mAHP. L-type current was not involved in the generation of either AHP but (with other HVA currents) contributes to the inward currents that regulate interspike intervals during repetitive firing. These data suggest different functional consequences for modulation of Ca2+ current subtypes.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
  • Calcium / metabolism*
  • Calcium Channel Blockers / pharmacology
  • Calcium Channels / classification
  • Calcium Channels / drug effects
  • Calcium Channels / physiology*
  • Cations, Divalent / pharmacology
  • Ion Transport / drug effects
  • Membrane Potentials / drug effects
  • Motor Cortex / cytology*
  • Nerve Tissue Proteins / classification
  • Nerve Tissue Proteins / drug effects
  • Nerve Tissue Proteins / physiology*
  • Nifedipine / pharmacology
  • Nimodipine / pharmacology
  • Patch-Clamp Techniques
  • Peptides / pharmacology
  • Pyramidal Cells / drug effects
  • Pyramidal Cells / physiology*
  • Somatosensory Cortex / cytology*
  • Spider Venoms / pharmacology
  • omega-Agatoxin IVA
  • omega-Conotoxin GVIA
  • omega-Conotoxins*


  • Calcium Channel Blockers
  • Calcium Channels
  • Cations, Divalent
  • Nerve Tissue Proteins
  • Peptides
  • Spider Venoms
  • omega-Agatoxin IVA
  • omega-Conotoxins
  • omega-conotoxin-MVIIC
  • Nimodipine
  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
  • omega-Conotoxin GVIA
  • Nifedipine
  • Calcium