Molecular approaches for colorectal cancer screening

Eur J Gastroenterol Hepatol. 1998 Mar;10(3):213-7. doi: 10.1097/00042737-199803000-00004.


Molecular genetic approaches for colorectal cancer screening using stool samples have the potential to be very specific, sensitive and cost effective. K-ras oncogene mutation, observed in about 50% of the colorectal tumours, serves as an ideal target for such an approach. Several studies have shown that K-ras gene mutations present in the tumours can be detected from the stool of patients with colorectal cancer. The challenge for clinical application, however, is to develop a simple method for robust PCR amplification and a reliable assay for K-ras gene mutation detection in the stool samples.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Colorectal Neoplasms / prevention & control*
  • Feces / cytology
  • Genes, ras / genetics*
  • Humans
  • Mass Screening / methods*
  • Molecular Biology / methods
  • Polymerase Chain Reaction