Cloning of a breakpoint cluster region on chromosome 14 in uterine leiomyoma

Cancer Lett. 1998 Apr 24;126(2):119-26. doi: 10.1016/s0304-3835(97)00478-3.


A recurrent reciprocal chromosomal translocation, t(12;14)(q15;q24) is frequently observed in uterine leiomyoma. Chromosome 12 breakpoints have been shown to occur in a region of approximately 150 kb that contains the gene for a high mobility group protein (HMGI-C). The breakpoint region on chromosome 14 has not been precisely defined. We have generated a contig of overlapping yeast artificial chromosome (YAC) clones approximately 3 Mb in size. Fluorescence in situ hybridization (FISH) analysis showed that this contig spanned the t(12;14) breakpoints in three uterine leiomyomas and that the breakpoints in these tumors occurred within a 1 Mb region. A 30 kb cosmid spanning one of the breakpoints was isolated to set the stage for identifying regions on chromosome 14 that may cause this region to be a preferential site for chromosomal translocation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomes, Artificial, Yeast / genetics
  • Chromosomes, Human, Pair 12 / genetics
  • Chromosomes, Human, Pair 14 / genetics*
  • Cloning, Molecular
  • DNA, Neoplasm / genetics*
  • Female
  • Genetic Markers
  • Humans
  • Karyotyping
  • Leiomyoma / genetics*
  • Polymerase Chain Reaction
  • Translocation, Genetic / genetics*
  • Uterine Neoplasms / genetics*


  • DNA, Neoplasm
  • Genetic Markers