Syntaxin-16, a putative Golgi t-SNARE

Eur J Cell Biol. 1998 Mar;75(3):223-31. doi: 10.1016/S0171-9335(98)80116-7.


Members of the syntaxin family of integral membrane proteins have recently been implicated as vesicle receptors on target membranes, coresponsible for the specificity of intracellular membrane traffic. So far, only a small number of different mammalian syntaxins have been identified. Here we report the cloning of three new human syntaxin cDNAs, presumably originating from alternative splicing of the same transcript. Syntaxin-16A and syntaxin-16B are identical, except that the latter contains an insertion of 21 amino acid residues. Syntaxin-16C is a truncated version of syntaxin-16A, lacking the C-terminal coiled-coil and hydrophobic regions characteristic for syntaxins. Database searches identified putative yeast, plant and nematode homologues of syntaxin-16, indicating that this protein is conserved through evolution, and syntaxin-16 belongs to a new subgroup of syntaxins. Epitope-tagged syntaxin-16A and syntaxin-16B were found to colocalize with the Golgi marker beta-COP, while syntaxin-16C was found in the cytosol. Syntaxin-16A associates posttranslationally with microsomes, and appears to be transported to the Golgi via the endoplasmic reticulum. The three syntaxin-16 forms may have differential roles in intracellular trafficking.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cricetinae
  • DNA, Complementary
  • Endoplasmic Reticulum / metabolism
  • Gene Expression
  • Golgi Apparatus / metabolism*
  • HeLa Cells
  • Humans
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Microsomes / metabolism
  • Molecular Sequence Data
  • Protein Processing, Post-Translational
  • Syntaxin 16


  • DNA, Complementary
  • Membrane Proteins
  • Syntaxin 16

Associated data

  • GENBANK/AF008935
  • GENBANK/AF008936
  • GENBANK/AF008937