Single-step purification of T7 RNA polymerase with a 6-histidine tag

Biotechniques. 1998 May;24(5):718-20. doi: 10.2144/98245bm03.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage T7 / enzymology*
  • Bacteriophage T7 / genetics*
  • DNA-Directed RNA Polymerases / genetics*
  • DNA-Directed RNA Polymerases / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Histidine / genetics*
  • Protein Engineering / methods
  • Recombinant Fusion Proteins / isolation & purification*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Recombinant Fusion Proteins
  • Histidine
  • DNA-Directed RNA Polymerases