Opposite roles of D1 and D5 dopamine receptors in locomotion revealed by selective antisense oligonucleotides

Neuroreport. 1998 Jan 5;9(1):1-5. doi: 10.1097/00001756-199801050-00001.

Abstract

Contralateral rotations induced by the D1-like agonist SKF 38393 in unilaterally 6-hydroxydopamine-lesioned rats were completely prevented by the administration of the D1-like antagonist SCH 23390. A similar result was obtained after intracerebroventricular administration of an antisense oligodeoxynucleotide for the D1 receptor (D1R-as). Contrariwise, administration of a D5R-as potentiated the effects of SKF 38393, showing a 60% increase in the rotational scores. Both effects were reversible upon cessation of D1R-as or D5R-as treatment and were also specific since rotational scores in rats treated with vehicle or with a randomly designed oligodeoxynucleotide were not modified. These results suggest that whereas D1 receptors play a facilitatory role in locomotion, D5 receptors exert an inhibitory effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / pharmacology
  • Animals
  • Benzazepines / pharmacology
  • Dopamine Agonists / pharmacology
  • Dopamine Antagonists / pharmacology
  • Female
  • Injections, Intraventricular
  • Motor Activity / physiology*
  • Oligonucleotides, Antisense*
  • Rats
  • Rats, Wistar
  • Receptors, Dopamine D1 / physiology*
  • Receptors, Dopamine D5
  • Rotation

Substances

  • Benzazepines
  • Dopamine Agonists
  • Dopamine Antagonists
  • Drd5 protein, rat
  • Oligonucleotides, Antisense
  • Receptors, Dopamine D1
  • Receptors, Dopamine D5
  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine