Construction of YAC-based mammalian artificial chromosomes

Nat Biotechnol. 1998 May;16(5):431-9. doi: 10.1038/nbt0598-431.


To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoantigens*
  • Cell Line
  • Cell Line, Transformed
  • Centromere / genetics
  • Centromere Protein B
  • Chromosomal Proteins, Non-Histone / chemistry
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomes / genetics*
  • Chromosomes, Artificial, Yeast / genetics*
  • Chromosomes, Human, Pair 21 / genetics
  • Cloning, Molecular
  • DNA, Satellite / chemistry
  • DNA, Satellite / genetics
  • DNA-Binding Proteins*
  • Genetic Vectors / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Kinetochores
  • Polymerase Chain Reaction
  • Telomere / genetics
  • Transfection


  • Autoantigens
  • CENPB protein, human
  • Centromere Protein B
  • Chromosomal Proteins, Non-Histone
  • DNA, Satellite
  • DNA-Binding Proteins
  • centromere protein C
  • centromere protein E