The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells

Exp Eye Res. 1998 Apr;66(4):411-9. doi: 10.1006/exer.1997.0442.


Advanced glycation endproducts have been implicated in a number of diabetic and aging changes. Some of these effects occur in part through induction of cytokines such as platelet-derived growth factor (PDGF), which is expressed by the retinal pigment epithelium (RPE). In this study, cultures of RPE were evaluated for PDGF expression after treatment with pentosidine, a well characterized advanced glycation endproduct. Northern analysis provided evidence for the increased expression of a 3.7 kb PDGF-B transcript over unstimulated controls in the established ARPE-19 cell line. Western analysis demonstrated increased PDGF-BB protein in conditioned medium compared to controls of ARPE-19 cells. In addition, two different early passage cultures of RPE showed increased PDGF-BB protein after pentosidine treatment compared to unstimulated controls. The enhanced production of PDGF-BB could play a role in the maintenance of the RPE-Bruch's membrane complex and influence changes associated with diabetes and aging.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anticoagulants / metabolism*
  • Arginine / analogs & derivatives*
  • Arginine / pharmacology
  • Becaplermin
  • Blotting, Northern
  • Blotting, Western
  • Cells, Cultured
  • Humans
  • Lysine / analogs & derivatives*
  • Lysine / pharmacology
  • Pigment Epithelium of Eye / metabolism*
  • Platelet-Derived Growth Factor / metabolism*
  • Proto-Oncogene Proteins c-sis


  • Anticoagulants
  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-sis
  • Becaplermin
  • Arginine
  • pentosidine
  • Lysine