Human cytomegalovirus matrix protein PP150 is efficiently presented as one of target antigens for cytotoxic T lymphocyte recognition

Chin Med J (Engl). 1997 May;110(5):397-400.

Abstract

Objective: To determine whether human cytomegalovirus (CMV) matrix protein PP150 is efficiently presented for CMV-specific CTL recognition.

Methods: Recombinant vaccinia virus (Vac. PP150) encoding human CMV structural matrix protein PP150 was constructed with vaccinia vector PSC11 and it was used to stimulate peripheral blond mononuclear cells from 5 CMV seropositive individuals.

Results: PP150-specific CTLs could be generated in all of them, which not only lysed Vac. PP150-infected fibroblasts, but also lysed CMV-infected targets. In the presence of RNA synthesis inhibitor Actinomycin D (Act D) or at very early stage of infection, PP150-specific CTL lysed CMV-infected targets as efficiently as in the absence of Act D or at late stage of infection.

Conclusions: PP150 exogenously introduced with the virus infection could be efficiently presented prior to viral DNA replication and PP150 is one of the major target antigens for CTL recognition.

MeSH terms

  • Antigens, Viral / immunology*
  • Cytomegalovirus / immunology*
  • Cytomegalovirus Infections / immunology
  • Cytomegalovirus Infections / virology
  • Cytotoxicity, Immunologic
  • Humans
  • Phosphoproteins*
  • Recombinant Proteins / immunology
  • Skin / virology
  • T-Lymphocytes / immunology*
  • Viral Matrix Proteins / analysis
  • Viral Matrix Proteins / immunology*

Substances

  • Antigens, Viral
  • Phosphoproteins
  • Recombinant Proteins
  • Viral Matrix Proteins
  • pp150 protein, Cytomegalovirus