Cloning and characterization of the gene encoding the primary sigma-factor of Campylobacter jejuni

FEMS Microbiol Lett. 1998 May 1;162(1):97-103. doi: 10.1111/j.1574-6968.1998.tb12984.x.


The rpoD gene encoding the primary sigma-factor of Campylobacter jejuni was amplified from genomic DNA with degenerate oligonucleotide primers. The complete gene encodes a polypeptide of 622 amino acids and has a deduced M(r) of 72.6 kDa. This polypeptide is 40% identical to the RpoD (sigma 70) protein of Escherichia coli and has 66% identity with the Helicobacter pylori RpoD protein. A C. jejuni sigma 70 promoter, not recognized by the E. coli sigma 70 factor, could be activated in this bacterium in the presence of the cloned C. jejuni RpoD protein.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Campylobacter jejuni / genetics*
  • Cloning, Molecular
  • DNA-Directed RNA Polymerases / genetics*
  • DNA-Directed RNA Polymerases / physiology
  • Gene Expression Regulation, Bacterial / genetics
  • Genes, Bacterial / genetics*
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sigma Factor / genetics*
  • Sigma Factor / physiology
  • Transcriptional Activation


  • Sigma Factor
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases

Associated data

  • GENBANK/AJ002379