Chloroplast development at low temperatures requires a homolog of DIM1, a yeast gene encoding the 18S rRNA dimethylase

Plant Cell. 1998 May;10(5):699-711. doi: 10.1105/tpc.10.5.699.

Abstract

Poikilothermic organisms require mechanisms that allow survival at chilling temperatures (2 to 15 degreesC). We have isolated chilling-sensitive mutants of Arabidopsis, a plant that is very chilling resistant, and are characterizing them to understand the genes involved in chilling resistance. The T-DNA-tagged mutant paleface1 (pfc1) grows normally at 22 degrees C but at 5 degrees C exhibits a pattern of chilling-induced chlorosis consistent with a disruption of chloroplast development. Genomic DNA flanking the T-DNA was cloned and used to isolate wild-type genomic and cDNA clones. The PFC1 transcript is present at a low level in wild-type plants and was not detected in pfc1 plants. Wild-type Arabidopsis expressing antisense constructs of PFC1 grew normally at 22 degrees C but showed chilling-induced chlorosis, confirming that the gene is essential for low-temperature development of chloroplasts. The deduced amino acid sequence of PFC1 has identity with rRNA methylases found in bacteria and yeast that modify specific adenosines of pre-rRNA transcripts. The pfc1 mutant does not have these modifications in the small subunit rRNA of the plastid.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acclimatization
  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / physiology*
  • Arabidopsis Proteins*
  • Base Sequence
  • Chloroplasts / physiology*
  • Cold Temperature
  • Conserved Sequence
  • DNA, Bacterial / genetics
  • Genes, Fungal
  • Genes, Plant*
  • Methyltransferases / genetics*
  • Methyltransferases / metabolism*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Plant Proteins / biosynthesis*
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Temperature

Substances

  • Arabidopsis Proteins
  • DNA, Bacterial
  • Plant Proteins
  • Saccharomyces cerevisiae Proteins
  • T-DNA
  • DIM1 protein, S cerevisiae
  • Methyltransferases
  • PFC1 protein, Arabidopsis

Associated data

  • GENBANK/AF051326