Universal cytochrome b primers facilitate intraspecific studies in molluscan taxa

Mol Mar Biol Biotechnol. 1998 Mar;7(1):7-11.

Abstract

We describe the construction of amplification primers designed to target a portion of the mitochondrial cytochrome b locus in a variety of molluscan taxa. Combinations of two sets of primers successfully amplified cytochrome b from several species of gastropods, bivalves, and cephalopods. Sequence analysis of these amplified products revealed nucleotide diversity in small samples within several of these taxa. We discuss the utility of these primer sets for studies of intraspecific phylogeny in mollusks and potentially other invertebrates.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arthropods / genetics
  • Base Sequence
  • Cytochrome b Group / genetics*
  • DNA Primers*
  • DNA, Mitochondrial / genetics*
  • Molecular Sequence Data
  • Mollusca / classification
  • Mollusca / genetics*
  • Polymerase Chain Reaction / methods
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • Cytochrome b Group
  • DNA Primers
  • DNA, Mitochondrial