Cloning, expression, and chromosomal localization of human long-chain fatty acid-CoA ligase 4 (FACL4)

Genomics. 1998 Apr 15;49(2):327-30. doi: 10.1006/geno.1998.5268.


Long-chain fatty acid-CoA ligase (also called fatty acid acyl-CoA synthetase) plays an essential role in lipid biosynthesis and fatty acid degradation. We report herein the cDNA cloning of the human long-chain fatty acid-CoA ligase 4 from a brain library. The cDNA encodes a functional long-chain fatty acid-CoA ligase that shows preference for arachidonic acid as substrate. We also studied the tissue distribution of gene expression by Northern hybridization. Human placenta, brain, testis, ovary, spleen, and adrenal cortex have the highest levels of expression of the long-chain fatty acid-CoA ligase 4, whereas the GI system has the lowest. Finally, this gene was localized to chromosome Xq23 in human by FISH analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Chromosome Mapping*
  • Cloning, Molecular / methods
  • Coenzyme A Ligases / biosynthesis
  • Coenzyme A Ligases / genetics*
  • Gene Expression / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • Repressor Proteins*
  • Saccharomyces cerevisiae Proteins*
  • X Chromosome / genetics


  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Coenzyme A Ligases
  • FAA2 protein, S cerevisiae
  • long-chain-fatty-acid-CoA ligase

Associated data

  • GENBANK/AF030555