1,25-Dihydroxyvitamin D3 inhibits tenascin-C expression in mammary epithelial cells

FEBS Lett. 1998 Apr 17;426(2):225-8. doi: 10.1016/s0014-5793(98)00348-2.


Tenascin-C is an extracellular matrix protein with growth-, invasion- and angiogenesis-promoting activities. Tenascin-C is upregulated in breast carcinoma and stromal cells, and in many other cell types during tumorigenesis. We demonstrate that tenascin-C RNA expression is inhibited by 1,25-dihydroxyvitamin D3 (1,25-D3) in a variety of mouse and human mammary epithelial cell lines exhibiting normal or malignant phenotype. In EpH4 cells, the inhibition is maximum 24 h after 1,25-D3 treatment and correlates with a dose-dependent reduction in the synthesis of tenascin-C protein. Furthermore, 1,25-D3 also abolishes the induction of tenascin-C by serum or the tumor promoter 12-O-tetradecanoyl phorbol 13-acetate. The inhibition of tenascin-C expression may be relevant for the anticancer activity of 1,25-D3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Breast Neoplasms / metabolism*
  • Calcitriol / pharmacology*
  • Carcinogens / pharmacology
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Mice
  • Neoplasm Proteins / biosynthesis
  • RNA, Messenger / genetics
  • RNA, Neoplasm / metabolism
  • Tenascin / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Cells, Cultured


  • Carcinogens
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Tenascin
  • Calcitriol
  • Tetradecanoylphorbol Acetate