Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51

Biochem Biophys Res Commun. 1998 May 8;246(1):222-7. doi: 10.1006/bbrc.1998.8546.

Abstract

A novel protein kinase related to the C. elegans serine/threonine kinase UNC-51 was cloned from mouse. The UNC-51-Like Kinase (ULK)1 is encoded by a cDNA of 1051 amino acids with calculated MW of 113 kDa. Comparison of the ULK1 and UNC-51 shows the highest conservation in the amino-terminal kinase domain, which is followed by a proline/serine-rich (PS) domain and a conserved carboxyl-terminal (C) domain. ULK1 mRNA is expressed in various tissues, and is mapped to mouse chromosome 5F and rat chromosome 12q16.3, by fluorescent in situ hybridization. HA-tagged ULK1 is expressed as a protein of approximately 150 kDa in COS7 cells and is auto-phosphorylated in vitro in its PS domain. We propose that ULK1, UNC-51 and a yeast protein kinase Apg1p comprise a novel subfamily of protein kinase, which is structurally conserved among eukaryotes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autophagy-Related Protein-1 Homolog
  • Base Sequence
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression
  • Helminth Proteins / genetics*
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Phosphorylation
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Sequence Homology, Amino Acid

Substances

  • Caenorhabditis elegans Proteins
  • DNA, Complementary
  • Helminth Proteins
  • RNA, Messenger
  • Protein Kinases
  • UNC-51 protein, C elegans
  • Autophagy-Related Protein-1 Homolog
  • Protein-Serine-Threonine Kinases
  • Ulk1 protein, mouse

Associated data

  • GENBANK/AF053756