Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: the phenylacetyl-CoA catabolon

Proc Natl Acad Sci U S A. 1998 May 26;95(11):6419-24. doi: 10.1073/pnas.95.11.6419.

Abstract

Fourteen different genes included in a DNA fragment of 18 kb are involved in the aerobic degradation of phenylacetic acid by Pseudomonas putida U. This catabolic pathway appears to be organized in three contiguous operons that contain the following functional units: (i) a transport system, (ii) a phenylacetic acid activating enzyme, (iii) a ring-hydroxylation complex, (iv) a ring-opening protein, (v) a beta-oxidation-like system, and (vi) two regulatory genes. This pathway constitutes the common part (core) of a complex functional unit (catabolon) integrated by several routes that catalyze the transformation of structurally related molecules into a common intermediate (phenylacetyl-CoA).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl Coenzyme A / genetics
  • Acetyl Coenzyme A / metabolism*
  • Amino Acid Sequence
  • Biodegradation, Environmental
  • Molecular Sequence Data
  • Mutation
  • Phenylacetates / metabolism*
  • Pseudomonas putida / metabolism*
  • Sequence Alignment

Substances

  • Phenylacetates
  • Acetyl Coenzyme A
  • phenylacetyl-coenzyme A
  • phenylacetic acid

Associated data

  • GENBANK/AF029714