Restricted usage of the T-cell receptor V beta repertoire in tonsillitis in association with palmoplantar pustulosis

Acta Derm Venereol. 1998 May;78(3):161-3. doi: 10.1080/000155598441431.


Focal infections such as chronic tonsillitis or dental caries occasionally play a role in the induction or exacerbation of palmoplantar pustulosis (PPP). Arthro-osteitis is sometimes a complication in severe cases of PPP. To study the effects of bacterial infection on the exacerbation of cutaneous lesions and arthralgia, we investigated the T-cell receptor V beta repertoire in peripheral blood mononuclear cells (PBMC) and tonsil tissue after tonsillectomy in 4 cases, who had chronic tonsillitis and a history of exacerbation of cutaneous lesions following a sore throat. First, serum levels of interleukin-6 (IL-6) and IL-8 were measured before and after tonsillectomy by enzyme-linked immunosorbent assay (ELISA). Second, 3H-TdR incorporation was used to examine the effects of the culture supernatant on the PBMC of the autologous patients, other PPP patients without tonsillitis and normal controls. T-cell receptor V beta repertoire was examined by the reverse transcriptase-polymerase chain reaction method. Results showed that IL-8 was significantly high in the serum and abundantly released from tonsillar lymphocytes, which may play a role in the accumulation of neutrophils in lesional skin. T-cell receptors V beta 6 and 12 were preferentially expressed on tonsillar lymphocytes, and V beta 4, 7, 9, 17 and 18 were detected relatively frequently. These data suggest that restricted usage of T-cell receptor V beta subsets may play a crucial role in the induction of tonsillitis associated with PPP.

MeSH terms

  • Adult
  • Culture Media, Conditioned / chemistry
  • Culture Media, Conditioned / pharmacology
  • Female
  • Gene Expression / genetics
  • Humans
  • Interleukin-6 / blood
  • Interleukin-8 / blood
  • Leukocytes, Mononuclear / drug effects
  • Lymphocyte Activation / immunology
  • Male
  • Middle Aged
  • Mitogens / immunology
  • Mitogens / pharmacology
  • Palatine Tonsil / cytology
  • Palatine Tonsil / metabolism
  • Palatine Tonsil / microbiology
  • Polymerase Chain Reaction
  • Psoriasis / complications
  • Psoriasis / immunology*
  • Psoriasis / microbiology
  • RNA / metabolism
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Staphylococcus aureus / cytology
  • Staphylococcus aureus / isolation & purification
  • Superantigens / physiology
  • T-Lymphocytes / chemistry
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology
  • Time Factors
  • Tonsillectomy
  • Tonsillitis / complications
  • Tonsillitis / immunology*
  • Tonsillitis / surgery


  • Culture Media, Conditioned
  • Interleukin-6
  • Interleukin-8
  • Mitogens
  • Receptors, Antigen, T-Cell, alpha-beta
  • Superantigens
  • RNA