Isolation of the zebrafish homologues for the tie-1 and tie-2 endothelium-specific receptor tyrosine kinases

Dev Dyn. 1998 May;212(1):133-40. doi: 10.1002/(SICI)1097-0177(199805)212:1<133::AID-AJA12>3.0.CO;2-8.


Several characteristics of the zebrafish embryo make it an attractive model in which to study the development of the cardiovascular system. The utility of the zebrafish as a model of mammalian vascular development will depend on the conservation of molecular and morphogenetic mechanisms of vessel growth. Here, we report the cloning of the zebrafish homologues of the endothelium-specific receptor tyrosine kinases tie-1 and tie-2. The Z tie-2 clone represents the first report of a full-length zebrafish endothelium-specific gene. The zebrafish tie family members have significant structural homology with their murine and human counterparts. In addition, like the murine tie-1 and tie-2 genes, expression was found predominantly in endothelial cells. At 24-hr postfertilization (HPF), Z tie-1 was expressed in all observed populations of endothelial cells. Interestingly, Z tie-2 exhibited a similar, although slightly more restricted, expression pattern. Taken together, these data strongly suggest that mechanisms of vascular development are highly conserved across species and that zebrafish will continue to be a useful model for the investigation of vertebrate embryonic vascular development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Endothelium, Vascular / embryology
  • Endothelium, Vascular / enzymology*
  • Gene Expression
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptor, TIE-1
  • Receptor, TIE-2
  • Receptors, Cell Surface / genetics*
  • Receptors, TIE
  • Sequence Homology, Amino Acid
  • Zebrafish
  • Zebrafish Proteins*


  • DNA, Complementary
  • Receptors, Cell Surface
  • Zebrafish Proteins
  • Receptor Protein-Tyrosine Kinases
  • Receptor, TIE-1
  • Receptor, TIE-2
  • Receptors, TIE
  • tek protein, zebrafish
  • tie1 protein, zebrafish

Associated data

  • GENBANK/AF053632