A lymphocyte-specific CC chemokine, secondary lymphoid tissue chemokine (SLC), is a highly efficient chemoattractant for B cells and activated T cells

Eur J Immunol. 1998 May;28(5):1516-23. doi: 10.1002/(SICI)1521-4141(199805)28:05<1516::AID-IMMU1516>3.0.CO;2-J.


Secondary lymphoid tissue chemokine (SLC) is a CC chemokine expressed mainly in lymph nodes, appendix and spleen, and specifically chemotactic for lymphocytes (Nagira et al., J. Biol. Chem. 1997. 272: 19518-19524). Here, we carried out transendothelial migration assays to determine the classes and subsets of lymphocytes migrating toward SLC. SLC attracted freshly isolated B cells with high efficiency and T cells modestly. Thus, SLC is the first CC chemokine with a strong chemotactic activity on fresh B cells. Among T cell types and subsets, SLC broadly attracted CD4+ and CD8+ cells, CD45RO- (naive) and CD45RO+ (memory) cells, and CD26high (activated) and CD26low- (resting) cells. SLC also attracted both L-selectin+ and L-selectin- subpopulations of various T cell subsets and B cells. Furthermore, mitogenic stimulation strongly enhanced migratory responses of T cells and B cells toward SLC. By in situ hybridization, SLC mRNA was detected in the cortical parafollicular regions (the T cell areas) of a lymph node and an appendix. Collectively, SLC may be a basic chemokine supporting homeostatic migration of a broad spectrum of lymphocytes into the secondary lymphoid tissues. SLC may also be involved in immune responses by inducing highly efficient migration of T and B cells following antigenic stimulation.

MeSH terms

  • Adult
  • B-Lymphocyte Subsets / immunology*
  • Chemokine CCL21
  • Chemokines, CC / blood
  • Chemokines, CC / genetics
  • Chemokines, CC / physiology*
  • Chemotaxis, Leukocyte / immunology*
  • Humans
  • Lymphocyte Activation* / drug effects
  • Lymphoid Tissue / cytology
  • Lymphoid Tissue / metabolism
  • Phytohemagglutinins / pharmacology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / metabolism
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism


  • CCL21 protein, human
  • Chemokine CCL21
  • Chemokines, CC
  • Phytohemagglutinins
  • RNA, Messenger