ChemR23, a putative chemoattractant receptor, is expressed in monocyte-derived dendritic cells and macrophages and is a coreceptor for SIV and some primary HIV-1 strains

Eur J Immunol. 1998 May;28(5):1689-700. doi: 10.1002/(SICI)1521-4141(199805)28:05<1689::AID-IMMU1689>3.0.CO;2-I.


Leukocyte chemoattractants act through a rapidly growing subfamily of G protein-coupled receptors. We report the cloning of a novel human gene encoding an orphan receptor (ChemR23) related to the C3a, C5a and formyl Met-Leu-Phe receptors, and more distantly to the subfamilies of chemokine receptors. ChemR23 transcripts were found to be abundant in monocyte-derived dendritic cells and macrophages, treated or not with LPS. Low expression could also be detected by reverse transcription-PCR in CD4+ T lymphocytes. The gene encoding ChemR23 was assigned by radiation hybrid mapping to the q21.2-21.3 region of human chromosome 12, outside the gene clusters identified so far for chemoattractant receptors. Given the increasing number of chemoattractant receptors used by HIV-1, HIV-2 and SIV as coreceptors, ChemR23 was tested in fusion assays for potential coreceptor activity by a range of viral strains. None of the tested HIV-2 strains made use of ChemR23 as a coreceptor, but several SIV strains (SIVmac316, SIVmac239, SIVmacl7E-Fr and SIVsm62A), as well as a primary HIV-1 strain (92UG024-2) used it efficiently. ChemR23 therefore appears as a coreceptor for immunodeficiency viruses that does not belong to the chemokine receptor family. It is also a putative chemoattractant receptor relatively specific for antigen-presenting cells, and it could play an important role in the recruitment or trafficking of these cell populations. Future work will be required to identify the ligand(s) of this new G protein-coupled receptor and to define its precise role in the physiology of dendritic cells and macrophages.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / chemistry
  • Base Sequence
  • Cell Line
  • Chromosomes, Human, Pair 2
  • Cloning, Molecular
  • Dendritic Cells / metabolism*
  • Gene Expression / immunology
  • HIV-1 / metabolism
  • Humans
  • Macrophages / metabolism*
  • Molecular Sequence Data
  • Monocytes / metabolism*
  • Receptor, Anaphylatoxin C5a
  • Receptors, Chemokine / biosynthesis*
  • Receptors, Chemokine / chemistry
  • Receptors, Chemokine / genetics
  • Receptors, Complement / chemistry
  • Receptors, Formyl Peptide
  • Receptors, HIV / biosynthesis*
  • Receptors, HIV / chemistry
  • Receptors, HIV / genetics
  • Receptors, Immunologic / chemistry
  • Receptors, Peptide / chemistry
  • Receptors, Virus / biosynthesis
  • Receptors, Virus / chemistry
  • Receptors, Virus / genetics
  • Simian Immunodeficiency Virus / metabolism*
  • Tumor Cells, Cultured


  • Antigens, CD
  • CMKLR1 protein, human
  • Receptor, Anaphylatoxin C5a
  • Receptors, Chemokine
  • Receptors, Complement
  • Receptors, Formyl Peptide
  • Receptors, HIV
  • Receptors, Immunologic
  • Receptors, Peptide
  • Receptors, Virus

Associated data

  • GENBANK/Y14838