Abstract
The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence-directed DNA curvature was identified in the promoter region of gyrA. The bend center was mapped and located between the -35 and -10 regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended -10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Cloning, Molecular
-
DNA Gyrase
-
DNA Topoisomerases, Type II / chemistry
-
DNA Topoisomerases, Type II / genetics*
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / genetics
-
Genes, Bacterial / genetics*
-
Molecular Sequence Data
-
Molecular Weight
-
Nucleic Acid Conformation
-
Promoter Regions, Genetic / genetics
-
Recombinant Fusion Proteins
-
Restriction Mapping
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
Streptococcus pneumoniae / enzymology
-
Streptococcus pneumoniae / genetics*
-
Transcription, Genetic / genetics
Substances
-
DNA, Bacterial
-
Recombinant Fusion Proteins
-
DNA Gyrase
-
DNA Topoisomerases, Type II