A quick method to extract DNA from paraffin-embedded tissues

Changgeng Yi Xue Za Zhi. 1998 Mar;21(1):63-6.


Background: The amplification power of the polymerase chain reaction (PCR) technique has had great impact in molecular analysis, and DNA extraction is a common requirement in retrospective studies utilizing PCR as a tool. Conventional methods used in deparaffinization, harvesting and purification of DNA from paraffin-embedded tissue are time consuming and cause a significant loss in the yield of DNA.

Methods: We utilized heating-melting-cooling removal of paraffin, digestion of the sample with proteinase K and purification of the extracted DNA by a microconcentrator. The products, after PCR amplification of the p53 gene exon 8, were used to make a comparison between our method and the conventional xylene-phenol-choloform method.

Results: The amplified products from our method were superior to that of the conventional method.

Conclusion: The method we propose has a better recovery of DNA and is more time efficient.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / isolation & purification*
  • Humans
  • Paraffin
  • Polymerase Chain Reaction*
  • Tissue Embedding


  • Paraffin
  • DNA