Use of FLP/FRT system to study Drosophila development

Methods. 1998 Apr;14(4):355-65. doi: 10.1006/meth.1998.0591.


Marked clones in mosaic animals have been used extensively to answer developmental questions in Drosophila. Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label mutant clones in both developing tissues and adult cuticle. Strains carrying these genetically marked FLP/FRT chromosomes have greatly enhanced our ability to study gene function in both germline and somatic Drosophila cells.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA Nucleotidyltransferases / genetics*
  • Drosophila / embryology
  • Drosophila / genetics*
  • Drosophila / growth & development*
  • Genes, Insect / genetics*
  • Genetic Markers / genetics
  • Insect Proteins / genetics*
  • Recombination, Genetic


  • Genetic Markers
  • Insect Proteins
  • DNA Nucleotidyltransferases
  • FLP recombinase