Targeted gene misexpression in chick limb buds using avian replication-competent retroviruses

Methods. 1998 Apr;14(4):407-20. doi: 10.1006/meth.1998.0595.

Abstract

The methods and applications for using avian replication-competent retroviruses to target gene misexpression in the developing limb bud of the chicken are described. These viruses provide the means to exploit the strengths of the chick as a model system in experimental embryology in conjunction with a genetic approach for ectopically expressing a gene of interest. The applications and strengths of the system are detailed. All the steps required to produce a virus carrying a transgene of interest and the methodologies behind designing and carrying out misexpression strategies are outlined, and some useful techniques for analyzing infected embryos are described.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Avian Sarcoma Viruses / genetics*
  • Base Sequence
  • Chick Embryo
  • Cloning, Molecular / methods
  • Gene Expression Regulation, Viral* / genetics
  • Gene Targeting / methods*
  • Limb Buds / metabolism*
  • Limb Buds / virology
  • Molecular Sequence Data
  • Replicon / genetics*