Determination of host specificity of cowpea miscellany Rhizobium spp. by nodABC-lacZ fusion

S Pandya; P Uchil; M Subramanian; A Desai

doi:10.1007/s002849900323

Determination of host specificity of cowpea miscellany Rhizobium spp. by nodABC-lacZ fusion

Curr Microbiol. 1998 Jun;36(6):361-4. doi: 10.1007/s002849900323.

Authors

S Pandya¹, P Uchil, M Subramanian, A Desai

Affiliation

¹ Department of Microbiology & Biotechnology Centre, Faculty of Science, M.S. University of Baroda, India.

PMID: 9608748
DOI: 10.1007/s002849900323

Abstract

Cowpea miscellany group of rhizobia are generally broad host range. Transconjugants of these cowpea rhizobial isolates having nodABC-lacZ fusion were monitored for flavonoid/root exudate-induced activation of the nod genes in terms of beta-galactosidase activity, thus determining the potential host range of the rhizobial isolates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyltransferases / genetics*
Amidohydrolases / genetics*
Artificial Gene Fusion*
Bacterial Proteins / genetics*
Lac Operon
N-Acetylglucosaminyltransferases / genetics*
Pisum sativum / microbiology*
Rhizobium / genetics*

Substances

Bacterial Proteins
nodB protein, Bacteria
Acyltransferases
NodA protein, Rhizobiales
N-Acetylglucosaminyltransferases
NodC protein, Rhizobiales
Amidohydrolases