High basal gastric acid secretion in somatostatin receptor subtype 2 knockout mice

Gastroenterology. 1998 Jun;114(6):1125-32. doi: 10.1016/s0016-5085(98)70417-2.


Background & aims: Somatostatin receptor subtype 2 (sst2) agonists inhibit gastric secretion. The role of sst2 in the regulation of acid secretion was assessed using sst2 knockout mice and urethane to induce somatostatin release.

Methods: Acid secretion was monitored every 10 minutes by gastric perfusion and backtitration of perfusates in fasted, urethane-anesthetized C57/129 sst2 (-/-) mice and wild-type (+/+) mice. The ileal vein was cannulated for drug injection. Intragastric pH and serum gastrin were monitored 1 hour after anesthesia without perfusion.

Results: Gastric pH values were lower in sst2 (-/-) mice (3.8 +/- 0.3) than in wild-type mice (7.1 +/- 0.1, P < 0.05), and there was no difference in gastrin levels. Basal acid output per 2 hours was 10-fold higher in sst2 knockout mice compared with wild-type mice. The gastrin antibody abolished the high basal acid secretion in sst2 (-/-) mice and had no effect in wild-type mice. The somatostatin antibody increased basal secretion by 4-fold in wild-type and had no effect in knockout mice. Somatostatin 14 or the sst2 agonist DC 32-87 inhibited pentagastrin-stimulated acid secretion in wild-type mice, but did not alter basal secretion in knockout mice.

Conclusions: These results indicate that sst2 is the main subtype whereby endogenous somatostatin suppresses gastric acid secretion through inhibition of gastrin action.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Gastric Acid / metabolism*
  • Gastric Mucosa / metabolism
  • Gastrins / blood
  • Gastrins / immunology
  • Hydrogen-Ion Concentration
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout / genetics*
  • Mice, Knockout / metabolism*
  • Pentagastrin / pharmacology
  • Receptors, Somatostatin / agonists
  • Receptors, Somatostatin / genetics*
  • Receptors, Somatostatin / physiology*
  • Somatostatin / analogs & derivatives
  • Somatostatin / immunology
  • Somatostatin / pharmacology
  • Somatostatin / physiology


  • Antibodies, Monoclonal
  • DC 32-87
  • Gastrins
  • Receptors, Somatostatin
  • Somatostatin
  • Pentagastrin