Abstract
A new Bacillus subtilis sporulation-specific gene, yhcN, has been identified, the expression of which is dependent on the forespore-specific sigma factor sigmaG and to a much lesser extent on sigmaF. A translational yhcN-lacZ fusion is expressed at a very high level in the forespore, and the protein encoded by yhcN was detected in the inner spore membrane. A yhcN mutant sporulates normally and yhcN spores have identical resistance properties to wild-type spores. However, the outgrowth of yhcN spores is slower than that of wild-type spores.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / genetics*
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Bacillus subtilis / growth & development
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Bacillus subtilis / physiology*
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Base Sequence
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Escherichia coli / genetics
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Molecular Sequence Data
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Mutagenesis, Insertional
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Promoter Regions, Genetic
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Sigma Factor / biosynthesis
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Sigma Factor / chemistry
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Sigma Factor / genetics
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Sigma Factor / physiology
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Spores, Bacterial / chemistry
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Spores, Bacterial / genetics
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Transcription Factors / biosynthesis
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / physiology
Substances
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Bacterial Proteins
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Sigma Factor
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Transcription Factors
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sporulation-specific sigma factors
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yhcN protein, Bacillus subtilis