Lack of a specific immune response against a recombinant capsid protein of Jaagsiekte sheep retrovirus in sheep and goats naturally affected by enzootic nasal tumour or sheep pulmonary adenomatosis

Vet Immunol Immunopathol. 1998 Feb 27;61(2-4):229-37. doi: 10.1016/s0165-2427(97)00149-9.


Enzootic nasal tumour (ENT) and sheep pulmonary adenomatosis (SPA) are two contagious adenocarcinomas of the respiratory tract of sheep and goats. Both diseases are associated with related, but distinct, type-D-retroviruses (ENTV and JSRV respectively). No evidence of circulating antibodies has been described in animals affected by either ENT or SPA using antigens from natural sources. We evaluated the usefulness of a recombinant JSRV capsid protein (JSRV-CA) as antigen to study the antibody responses of animals naturally affected by ENT or SPA, using immunoblotting. Positive reactions were detected in the sera of both affected and unaffected sheep and goats. The reactivity was abolished completely by absorption with the GST fusion partner but not by JSRV-CA, suggesting that it was not specific. The results support prior observations indicating that sheep and goats infected by JSRV and ENTV do not develop specific humoral responses to these retroviruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Antibody Specificity
  • Antigens, Viral / genetics
  • Betaretrovirus / genetics
  • Betaretrovirus / immunology
  • Blotting, Western
  • Capsid / genetics
  • Capsid / immunology*
  • Goat Diseases / diagnosis
  • Goat Diseases / immunology*
  • Goats
  • Nose Neoplasms / diagnosis
  • Nose Neoplasms / immunology
  • Nose Neoplasms / veterinary*
  • Pulmonary Adenomatosis, Ovine / diagnosis
  • Pulmonary Adenomatosis, Ovine / immunology*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Retroviridae Proteins / genetics
  • Retroviridae Proteins / immunology*
  • Sheep
  • Sheep Diseases / diagnosis
  • Sheep Diseases / immunology*


  • Antibodies, Viral
  • Antigens, Viral
  • Recombinant Fusion Proteins
  • Retroviridae Proteins