A New Homeodomain-Leucine Zipper Gene From Arabidopsis Thaliana Induced by Water Stress and Abscisic Acid Treatment

Plant Mol Biol. 1998 May;37(2):377-84. doi: 10.1023/a:1006084305012.

Abstract

We report the isolation and characterization of a new homeobox gene from Arabidopsis thaliana using a polymerase chain reaction (PCR) cloning strategy. The full-length cDNA, designated Athb-12, encodes a protein of 235 amino acids. It contains the conserved DNA binding domain and the leucine zipper motif, characteristic of the homeodomain-leucine zipper family of transcription factors. The deduced amino acid sequence of Athb-12 shows over 80% identity to the Arabidopsis Athb-7 in the homeodomain (82%) and the leucine zipper motif (80%) of the proteins. However, outside the homeodomain and the leucine zipper motif, the homology is significantly lower. RNA analysis identified only one 0.96 kb transcript consistent with the size of Athb-12 cDNA. The Athb-12 transcript was detected in stem, leaf, flower and root as well as in seedlings. Treatment with water stress and exogenous abscisic acid (ABA) resulted in the accumulation of Athb-12 mRNA, similar to that of Athb-7. However, the time course of the Athb-12 response to ABA differed from that of Athb-7, suggesting that both genes, in response to ABA, are regulated in different manners. Taken together, these data suggest that Athb-12 and Athb-7 are members of a related gene family involved in the plant's response to water stress.

MeSH terms

  • Abscisic Acid / pharmacology*
  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Cloning, Molecular
  • Desiccation
  • Gene Expression Regulation, Plant / drug effects
  • Gene Expression Regulation, Plant / physiology*
  • Genes, Homeobox / genetics
  • Genes, Plant / genetics
  • Homeodomain Proteins / genetics*
  • Leucine Zippers*
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*
  • Water

Substances

  • Arabidopsis Proteins
  • Homeodomain Proteins
  • RNA, Messenger
  • RNA, Plant
  • Transcription Factors
  • atHB-12 protein, Arabidopsis
  • Water
  • Abscisic Acid

Associated data

  • GENBANK/AF001949