Radioimmunoassay of free genistein in human serum

J Steroid Biochem Mol Biol. 1998 Mar;64(5-6):261-8. doi: 10.1016/s0960-0760(97)00199-4.

Abstract

Two radioimmunoassay (RIA) systems for genistein have been established, based on polyclonal antibodies against genistein-4'-O-(carboxymethyl)ether-bovine serum albumin and genistein-7-O-(carboxymethyl)ether-bovine serum albumin conjugates. The sensitivities of assays were 4.44 and 10.4 fmol (1.2 and 2.8 pg)/tube, respectively, the intraassay coefficients of variation ranged from 3.54 to 9.30%, the interassay C.V. varied from 6.72 to 19.7%, depending on the type of method and on genistein concentration. The cross-reactivities with other chemically related compounds (with exception of genistein derivatives at the position used for construction of the immunogen) were 5.5 and 6.1% for daidzein and 3.9 and 0.04% for formononetin in RIAs using reagents prepared through positions 4'- and 7- of genistein, respectively. The method was used for measurement of genistein levels in 26 omnivore subjects and in three volunteers after consumption of a meal prepared from 125 g of cooked whole soybeans. The values obtained in ether extracts from human sera were almost identical for both RIA systems, indicating that both RIAs measure the same entity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cross Reactions
  • Genistein / blood*
  • Humans
  • Iodine Radioisotopes / metabolism
  • Isoflavones / blood
  • Radioimmunoassay / methods*
  • Sensitivity and Specificity
  • Soybeans / metabolism

Substances

  • Iodine Radioisotopes
  • Isoflavones
  • Genistein